Protein_Domain

Part:BBa_K4430002:Design

Designed by: Ruyi Shi   Group: iGEM22_GYHS   (2022-09-20)


CBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 90
    Illegal AgeI site found at 72
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In general, endolysin-targeting Gram-positive bacteria are composed of N-terminal enzymatic activity domains (EADs) with bacterial cell-lysing capability and C-terminal cell-binding domain (CBD) for adhering to the bacterial cell wall. Because the total amount of CBD binding sites on a single cell can be 107 or more, the binding capability of CBD is comparable to that of an antibody. Researchers had shown that CBD of bacteriophage P108 binds strongly to the cell surfaces of SA. In order to separate SA from food samples, CBD was coupled with carboxyl magnetic beads, and then SA could be separated from the samples through magnets. At last, SA was detected combined with paper-based sensor.


Source

The source of the part will be its sequence which was retrieved from NCBI.

References